Protective Effect of Immunization with Heat-Labile Enterotoxin in Gnotobiotic Rats Monocontaminated with Enterotoxigenic Escherichia coli
نویسندگان
چکیده
منابع مشابه
Immunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli.
Rats immunized with Escherichia coli heat-labile (LT) enterotoxin, either in the form of the holotoxin derived from a transformed K-12 strain or the polymyxin-release form obtained from human strains which produce LT toxin alone (LT+/ST- [ST is heat-stable toxin)] or together with ST toxin (LT+/ST+), were challenged with viable organisms of 10 different serotypes, 5 LT+/ST- and 5 LT+/ST+. The s...
متن کاملTranscutaneous immunization using colonization factor and heat-labile enterotoxin induces correlates of protective immunity for enterotoxigenic Escherichia coli.
Enterotoxigenic Escherichia coli (ETEC) diarrheal disease is a worldwide problem that may be addressed by transcutaneous delivery of a vaccine. In several human settings, protective immunity has been associated with immune responses to E. coli colonization factors and to the heat-labile toxin that induces the diarrhea. In this set of animal studies, transcutaneous immunization (TCI) using recom...
متن کاملIntestinal Strangulation in Escherichia coli-Monocontaminated Gnotobiotic Rats.
The natural course of ischemic and hemorrhagic intestinal strangulation in previously germ-free rats individually monocontaminated with one of five separate strains of Escherichia coli was investigated. In addition, the effect of intraintestinal homologous heparinized blood upon the course of ischemic intestinal strangulation in E. coli-monocontaminated rats was studied. It was found that (i) E...
متن کاملHeat - labile Enterotoxin of Escherichia coli
Heat-labile enterotoxin (LT) was obtained in large quantities (several-gram amounts) and great purity from Escherichia coli C600 carrying the LT-coding multicopy plasmid EWD299. By growing this strain on a medium that allows high cell densities in the early stationary phase, we increased the net LT production per milliliter by a factor of 200, compared to natural porcine enterotoxigenic E. coli...
متن کاملEscherichia coli Heat - labile Enterotoxin NUCLEOTIDE SEQUENCE
We report the complete DNA sequence of the Escherichia coli elt A gene, which codes for the A subunit of the heat-labile enterotoxin, LT. The amino acid sequence of the LT A subunit has been deduced from the DNA sequence of elt A. The LT A subunit starts with methionine, ends with leucine, and comprises 264 amino acids. The computed molecular weight of LT A is 29,673. The A subunit of cho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1980
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.28.1.163-170.1980